Physicochemical characteristics of oil palm frond and application of CMF Hydrogel as a natural encapsulant for probiotic

Abstract Oil palm solid waste from Indonesia’s large oil plantations has enormous potential to meet various human needs. Lb. fermentum InaCC B 1295 (LFB1295) was tested in vitro for viability, acid and bile tolerance, safety assessment, antioxidant activities as a probiotic. The physiochemical characteristics of frond (OPF) cellulose microfiber (CMF) were also examined. OPF mainly consisted carbohydrates, particularly fiber, followed by ash, protein, fat. major components fiber cellulose, lignin, hemicellulose. crystal index the 93.4%, according an X-ray diffraction examination. vibrations that stretch cellulose’s -OH group discovered via FTIR analysis at 3420.05 cm-1. viability LFB1295 maintained 9.99 log CFU/g CMF OPF. persistence under bile-containing conditions low pH characterized decrease cell number 2.03 1.56 CFU/mL, respectively. Based on its ability repel hydrogen peroxide, neutralize DPPH radicals, actively hydroxyl radicals. LFB1295, encapsulated hydrogel OPF, good characteristics. This fact is demonstrated value Hydroxyl radical scavenging activity, which 78.43%, capacity scavenge inhibition IC50 47.28%. Encapsulated passed all tests.